Hamilton Syringe Pump ML600

Hamilton ML600 pumps connected via serial (RS-232) cables are supported in flowchem via the ML600 device type. As for all flowchem devices, the virtual instrument can be instantiated via a configuration file that generates an openAPI endpoint. For a standard (single syringe) pump model, two components will be available: one pump and one valve. Pumps with multiple syringes are not currently supported.

Configuration

Configuration sample showing all possible parameters:

[device.my-ml600-pump]  # This is the pump identifier
type = "ML600"
port = "COM1"  # This will be /dev/tty* under linux/MacOS
address= 1  # If multiple devices are daisy chained, number in the chain 1=first...
syringe_volume = "1 ml"  # If the wrong syringe volume is set, the flow rate will be wrong.

Note

Serial connection parameters Note, further parameters for the serial connections (i.e. those accepted by serial.Serial) such as baudrate, parity, stopbits and bytesize can be specified. However, it should not be necessary as the following values (which are the default for the instrument) are automatically used:

  • baudrate 9600

  • parity even

  • stopbits 1

  • bytesize 7

API methods

Once configured, a flowchem ML600 object will expose the following commands:

PUT /hplc/run

Run

Run one analysis on the instrument. The sample name has to be set in advance via sample-name.

Note that it takes at least 2 sec until the run actually starts (depending on instrument configuration). While the export of the chromatogram in e.g. ASCII format can be achieved programmatically via the CLI, the best solution is to enable automatic data export for all runs of the HPLC as the chromatogram will be automatically exported as soon as the run is finished.

Status Codes

  • 200 OK – Successful Response

PUT /hplc/method

Set Method

Sets the HPLC method (i.e. a file with .MET extension) to the instrument.

Make sure to select ‘Send Method to Instrument’ option in Method Sending Options dialog in System Configuration.

Query Parameters

  • method_name (string) – (Required)

Status Codes
PUT /hplc/sample-name

Set Sample Name

Sets the name of the sample for the next run.

Query Parameters

  • sample_name (string) – (Required)

Status Codes
PUT /hplc/exit

Exit

Exit Clarity Chrom.

Status Codes

  • 200 OK – Successful Response

Device detection

Lab PCs often have several devices connected via serial ports. ML600 pumps can be auto-detected via the flowchem-autodiscover command-line utility. After having installed flowchem, run flowchem-autodiscover to create a configuration stub with all the devices that can be auto-detected on your PC.

Further information

For further information about connection of the pump to the controlling PC, daisy-chaining via RJ-12 cables etc. please refer to the manufacturer manual.